Description

Recombinant Mouse USAG-1 (SOSTDC1) Protein

The Recombinant Mouse USAG-1 (SOSTDC1) Protein is an E. coli-derived glycoprotein, expressed from Phe24–Ser206 with an N-terminal Met. This high-purity (>95%) protein is validated for bioactivity and functional assays, making it ideal for research on BMP/Wnt signaling, tooth and bone development, and developmental biology studies.

Key Features & Benefits of Mouse USAG-1

• High-purity recombinant protein (>95% by SDS-PAGE, Coomassie® Blue staining)
• Validated bioactivity ensures reliable and reproducible experimental results
• Derived from E. coli expression system for consistent lot-to-lot quality
• Low endotoxin level: <0.10 EU per µg of protein (LAL method)
• Lyophilized format with long-term stability (-20 to -70 °C)
• Functional in BMP/Wnt signaling, tooth and bone development research
• Unconjugated, suitable for downstream assays, ELISA, and protein interaction studies

Mouse USAG-1 Specifications

Background

USAG1 (Uterine Sensitization-Associated Gene 1), also known as SOSTDC1, WISE, or Ectodin, is a secreted monomeric glycoprotein and a member of the sclerostin family of BMP antagonists. It plays a critical role in regulating BMP and Wnt signaling during kidney, tooth, and mammary tissue development. USAG1 contains a cystine knot domain, binds BMP-2, -4, -6, -7, and LRP5/6, and modulates apoptosis and organogenesis. Mouse USAG1 shares high sequence identity with human (97%) and rat (99.5%) orthologs.

Applications of The Mouse USAG-1

• Functional ELISA for BMP/Wnt pathway studies
• Tooth morphogenesis and bone development research
• In vitro bioactivity and ligand-binding assays
• Developmental biology and organogenesis studies

Formulation & Reconstitution

Lyophilized from a 0.2 µm filtered HCl solution. Reconstitute at 250 µg/mL in 4 mM HCl. Follow sterile handling procedures and avoid repeated freeze-thaw cycles.

Shipping & Storage

• Shipped at ambient temperature.
• Long-term storage: Lyophilized -20 to -70 °C (12 months).
• Short-term storage: Reconstituted 2–8 °C (1 month) or -20 to -70 °C (3 months).

Technical Datasheet:

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Download Safety Datasheet (SDS) :

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Certificate of Analysis

To receive a Certificate of Analysis, please send us the lot number to info@dentinova.co.uk after purchasing.

References

• Laurikkala, J. et al., Dev. Biol. 264:91, 2003.
• Yanagita, M. et al., Biochem. Biophys. Res. Commun. 316:490, 2004.
• Lintern, K.B. et al., J. Biol. Chem. 284:23159, 2009.
• Ellies, D.L. et al., PLoS One 9:e96257, 2014.
• Additional references on BMP/Wnt signaling and USAG1/SOSTDC1 function are available upon request.

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