325.00£
Brand: STEMCELL Technologies
Product: DisCoZyme 1, ACF
Origin: Canada
Size: 50mg
DisCoZyme 1 ACF is an animal component-free collagenase/dispase blend for efficient tissue dissociation and primary cell isolation. Ideal for stem cell, neural, and respiratory research, this enzymatic solution preserves cell viability and membrane integrity, offering reproducible results for multiple species and experimental workflows.
DisCoZyme 1, ACF Collagenase/Dispase Blend is a high-performance, animal component-free enzyme solution specifically designed for efficient tissue dissociation and primary cell isolation in advanced biomedical research. This collagenase and dispase blend combines powerful collagen-degrading activity with gentle proteolytic properties to preserve cell viability and membrane integrity, making it ideal for stem cell research, neural tissue isolation, and respiratory biology studies.
DisCoZyme 1 ACF is manufactured without animal-derived components, minimizing the risk of introducing pathogens into sensitive cell culture experiments. This makes it ideal for laboratories requiring high biosafety standards, including clinical research, regenerative medicine, and advanced stem cell workflows. The enzyme blend supports consistent results while maintaining the sterility and safety of experimental protocols.
The product contains collagenase from Clostridium histolyticum and dispase from Bacillus polymyxa, creating a synergistic effect that efficiently digests connective tissue while gently separating cells. Collagenase targets native collagen fibrils, whereas dispase cleaves peptide bonds at the N-terminal side of non-polar amino acids, preserving delicate cell membranes. This combination ensures maximal cell yield and viability for downstream applications.
DisCoZyme 1 ACF contains ≥250 collagenase units/mg and ≥1000 caseinase units/mg of dry weight, providing consistent and reproducible enzymatic activity across multiple experiments. The carefully balanced activity allows for efficient tissue dissociation while maintaining functional properties of stem cells and primary cells, which is essential for cell culture, regenerative studies, and molecular biology research.
This collagenase/dispase blend has been extensively used for neural stem and progenitor cell isolation and airway/lung tissue digestion. Its mild proteolytic activity ensures cells remain viable and functional, making it suitable for respiratory research, neurobiology studies, and primary cell isolation workflows. Researchers can rely on DisCoZyme 1 ACF for delicate tissues requiring gentle yet effective enzymatic digestion.
DisCoZyme 1 ACF supports tissue dissociation from human, mouse, rat, and non-human primate samples, offering versatility for a wide range of experimental models. It is commonly used in mesenchymal stem cell research, tissue dissociation for primary cultures, and neural tissue studies, ensuring broad applicability in modern biomedical and translational research laboratories.
By combining collagenase and dispase in an animal component-free formulation, DisCoZyme 1 ensures high cell viability, minimal apoptosis, and preservation of cellular function. This is especially critical for stem cell isolation workflows in which cells need to retain regenerative potential. DisCoZyme 1 ACF is a trusted solution for both academic and industrial research applications.
Store DisCoZyme 1 ACF at recommended temperatures to maintain activity. Avoid repeated freeze-thaw cycles, and follow standard tissue dissociation protocols for optimal results. The enzyme blend is shipped with appropriate safety and storage instructions to maintain long-term stability and reproducibility.
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