65.00£
Brand: STEMCELL Technologies
Product: Collagenase Type 1 (0.25%)
Origin: Canada
Size: 5ml.
Collagenase Type I (0.25%) is a ready-to-use enzymatic solution designed for efficient stem cell and primary cell isolation. Derived from Clostridium histolyticum, it digests collagen in connective tissues to generate viable cell suspensions. Ideal for mesenchymal stem cell research, embryoid body dissociation, and collagen gel solubilization in advanced cell culture workflows.
Collagenase Type I (0.25%) is a high-quality enzymatic solution specifically formulated for efficient tissue digestion and stem cell isolation in laboratory research applications. Derived from Clostridium histolyticum, this enzymatic preparation contains a mixture of collagen-degrading enzymes and auxiliary proteases that effectively break down native collagen fibers in connective tissues. The result is the generation of viable and high-quality cell suspensions suitable for a wide range of cell culture and regenerative research workflows.
This ready-to-use 5 ml solution is optimized for researchers working in mesenchymal stem cell isolation, embryoid body dissociation, and collagen matrix digestion. By selectively degrading collagen within extracellular matrices, Collagenase Type I enables efficient release of intact cells from complex tissues while preserving cell viability and functionality.
The enzymatic activity present in this preparation includes collagenase, caseinase, clostripain, and trypsin-like proteases, which collectively facilitate the breakdown of collagen networks and associated extracellular matrix proteins. This multi-enzyme activity ensures reliable tissue dissociation across a variety of biological samples.
Efficient collagen digestion
Collagenase Type I specifically targets collagen fibers within connective tissue, enabling effective breakdown of the extracellular matrix and efficient cell release.
Optimized concentration (0.25%)
The enzyme concentration is carefully calibrated to provide effective tissue digestion while maintaining high cell viability during isolation procedures.
Ideal for stem cell research
Widely used for isolating mesenchymal stem cells (MSCs) and other primary cells from tissues such as adipose tissue, dental pulp, bone marrow, and connective tissues.
Ready-to-use formulation
Supplied as a prepared solution containing collagenase in phosphate-buffered saline (PBS) with fetal bovine serum (FBS) to support enzymatic stability and improve digestion performance.
Broad species compatibility
Suitable for use with samples from human, mouse, rat, non-human primates, and other model organisms, making it versatile for multiple research environments.
Reliable enzymatic performance
Produced from Clostridium histolyticum, a well-established source of collagenase enzymes widely used in biomedical and cell culture laboratories.
Collagenase Type I (0.25%) plays a critical role in numerous cell culture and regenerative medicine workflows, particularly where tissue digestion and cell extraction are required.
One of the most common uses of this enzyme solution is the isolation of mesenchymal stem and progenitor cells from connective tissues. By breaking down collagen matrices, the enzyme releases stem cells while preserving their viability for downstream culture and expansion.
Researchers frequently use collagenase to generate single-cell suspensions from tissue samples. This process is essential for cell culture experiments, flow cytometry, cell sorting, and molecular biology analysis.
In pluripotent stem cell research, collagenase assists in the controlled dissociation of embryoid bodies (EBs) to allow further differentiation studies or cell expansion.
Collagenase Type I can also digest collagen-based hydrogels and matrices, enabling recovery of embedded cells or evaluation of matrix remodeling in tissue engineering experiments.
This Collagenase Type I preparation contains:
Collagenase Type I (0.25%)
Phosphate-Buffered Saline (PBS)
Fetal Bovine Serum (FBS) – 20%
The inclusion of FBS helps stabilize the enzyme mixture and improves digestion performance during tissue dissociation protocols.
Enzyme Source: Clostridium histolyticum
Subtype: Enzymatic tissue digestion reagent
Volume: 5 ml solution
Primary Applications: Cell culture, stem cell isolation, tissue dissociation
Compatible Species: Human, mouse, rat, non-human primates, and other research organisms
Collagenase Type I is widely used across several biomedical research disciplines, including:
Mesenchymal stem and progenitor cell research
Regenerative medicine
Tissue engineering
Cell culture and primary cell isolation
Pluripotent stem cell research
In particular, this enzyme is essential for workflows involving cell sourcing and isolation, where gentle yet effective tissue digestion is required.
Reliable tissue digestion is a critical step in successful cell culture and stem cell research. Collagenase Type I (0.25%) provides a balanced enzymatic formulation that ensures efficient collagen degradation, high cell yield, and preserved cellular integrity.
Its ready-to-use format, broad compatibility with multiple species, and consistent enzymatic activity make it a trusted reagent for laboratories performing stem cell isolation, extracellular matrix digestion, and tissue dissociation procedures.
Whether used in mesenchymal stem cell extraction, embryoid body processing, or collagen matrix digestion, this collagenase solution provides dependable performance for demanding research applications.
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